Immunization of susceptible BALB/c mice against Leishmania braziliensis. II. Use of temperature-sensitive avirulent clones of parasite for vaccination purposes |
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| Authors: | R M Gorczynski |
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| Affiliation: | 1. Veterinary Services Department, Kenya Wildlife Service, Nairobi, Kenya;2. Department of Biochemistry and Biotechnology, Kenyatta University, Nairobi, Kenya;3. Institute of Primate Research, National Museums of Kenya, Kenya;1. Department of Bioinformatics & Structural Biology, Indian Institute of Advanced Research, Koba Institutional Area, Gandhinagar 382007, Gujarat, India;2. Institute of Science, Nirma University, Gota, Ahmedabad, Gujarat 382481, India;3. Department of Biotechnology, Panjab University, South Campus, Chandigarh 160014, Panjab, India;4. School of Life Sciences, Central University of Gujarat, Gandhinagar 382030, Gujarat, India;1. Department of Psychiatry and Behavioral Neuroscience, University of Cincinnati College of Medicine, Cincinnati, OH 45237, USA;2. Graduate Program in Neuroscience, University of Cincinnati, Cincinnati 45237, USA;3. Department of Psychiatry & Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA |
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| Abstract: | Avirulent clones of Leishmania braziliensis were produced by treatment of parental parasite stock with the mutagen N-methyl-N'-methyl-N'-nitro-N-nitrosoguanidine and selection for growth at 19 degrees C. These clones have a clear preference for infection and growth in vitro at 19 degrees C rather than the normal 28 degrees C. In addition, they can be used to vaccinate naive mice successfully against growth of the parental parasite clones. Immunity can be adoptively transferred from vaccinated mice by Lyt-1+ cells from immunized animals. |
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