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A photoinduced cleavage of DNA useful for determining T residues. |
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| Authors: | A Simoncsits I T r k |
| Affiliation: | A Simoncsits and I Török |
| Abstract: | Irradiation of 5'-[32P]-phosphate labeled DNA fragments with ultraviolet light in the presence of primary amines followed by piperidine treatment resulted in base-specific cleavage of the DNA chain at T residues, accompanied by a less intensive G reaction. This simple, T greater than G cleavage offers an alternative method for determining T residues in chemical DNA sequencing. |
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