Isolation, characterization and cross-species amplification of polymorphic microsatellite loci in Asclepias (Apocynaceae) |
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| Authors: | Robin L O’Quinn Mark Fishbein |
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| Institution: | (1) Department of Biology, Eastern Washington University, 258 Science Building, Cheney, WA 99004, USA;(2) Department of Biology, Portland State University, P.O. Box 751, Portland, OR 97207, USA; |
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| Abstract: | Ten polymorphic loci were isolated and characterized from the milkweed, Asclepias syriaca L., of North America. These loci successfully cross-amplified in A. exaltata L. Polymorphism ranged from two to 16 alleles per locus per species in 68 individuals of A. syriaca and 56 individuals of A. exaltata. Expected heterozygosities ranged from 0.017 to 0.851 and significant deviations from Hardy–Weinberg equilibrium were found
for two and three loci in A. syriaca and A. exaltata, respectively. No linkage disequilibrium was detected. These markers should prove useful for assessing population genetic
structure and interspecific gene flow in these and other species of Asclepias. |
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