Characterization of the <Emphasis Type="Italic">GHR</Emphasis> gene genetic variation in Chinese indigenous goat breeds |
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Authors: | W L Bai C Y Zhou Y Ren R H Yin W Q Jiang S J Zhao S C Zhang B L Zhang G B Luo Z H Zhao |
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Institution: | (1) College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110161, China;(2) Animal Health Supervision and Management Bureau of Liaoning Province, Shenyang, 110003, China;(3) Division of Agriculture, Public Teaching Center, Jilin University, Changchun, 130062, China;(4) Institute of Biotechnology, Animal Science Research Academy of Sichuan Province, Chengdu, 610066, China;(5) College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062, China |
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Abstract: | The aim of the present work was to investigate single nucleotide polymorphism (SNP) of growth hormone receptor (GHR) gene exon 10, characterize the genetic variation in three Chinese indigenous goat breeds, and search for its potential association
with cashmere traits. In this study, a polymerase chain reaction-single strand conformation polymorphism (PCR–SSCP) protocol
has been developed for rapid genotyping of the GHR gene in goats. One hundred seventy-eight goats from Liaoning Cashmere (96), Inner Mongolia White Cashmere (40), and Chengdu
Grey (42) breeds in China were genotyped at GHR locus using the protocol developed. In all goat breeds investigated, a SNP in exon 10 of GHR gene has been identified by analyzing genomic DNA. The polymorphism consists of a single nucleotide substitution A → G, resulting
in two alleles named, respectively, A and G based on the nucleotide at the position. The allele A was found to be more common
in the animals investigated, and seems to be more consistent with cattle and zebu at this polymorphic site found in goats.
The Hardy–Weinberg equilibrium of genotype distributions of GHR locus was verified in Liaoning Cashmere, and Inner Mongolia White Cashmere breeds. According to the classification of polymorphism
information content (PIC), Chengdu Grey was less polymorphic than Liaoning Cashmere and Inner Mongolia White Cashmere breeds at this locus. The phylogenetic
tree of different species based on the nucleotide sequences of GHR gene exon 10 is generally in agreement with the known species relationship. No significant association was found between
the polymorphism revealed and the cashmere traits analyzed in present work. |
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