The C-type Arabidopsis thioredoxin reductase ANTR-C acts as an electron donor to 2-Cys peroxiredoxins in chloroplasts |
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Authors: | Moon Jeong Chan Jang Ho Hee Chae Ho Byoung Lee Jung Ro Lee Sun Yong Jung Young Jun Shin Mi Rim Lim Hye Song Chung Woo Sik Yun Dae-Jin Lee Kyun Oh Lee Sang Yeol |
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Institution: | Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea. |
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Abstract: | 2-Cys peroxiredoxins (Prxs) play important roles in the antioxidative defense systems of plant chloroplasts. In order to determine the interaction partner for these proteins in Arabidopsis, we used a yeast two-hybrid screening procedure with a C175S-mutant of Arabidopsis 2-Cys Prx-A as bait. A cDNA encoding an NADPH-dependent thioredoxin reductase (NTR) isotype C was identified and designated ANTR-C. We demonstrated that this protein effected efficient transfer of electrons from NADPH to the 2-Cys Prxs of chloroplasts. Interaction between 2-Cys Prx-A and ANTR-C was confirmed by a pull-down experiment. ANTR-C contained N-terminal TR and C-terminal Trx domains. It exhibited both TR and Trx activities and co-localized with 2-Cys Prx-A in chloroplasts. These results suggest that ANTR-C functions as an electron donor for plastidial 2-Cys Prxs and represents the NADPH-dependent TR/Trx system in chloroplasts. |
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Keywords: | Chloroplast 2-Cys peroxiredoxin Electron donor Hybrid protein NADPH-dependent thioredoxin reductase isotype C Yeast two-hybrid assay |
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