Hydroxyl free radicals generated by vanadyl[IV] induce cell blebbing in mitotic human Chang liver cells |
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Authors: | Boon-Huat Bay Kwok-Hung Sit Ramanujam Paramanantham Yee-Gek Chan |
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Affiliation: | (1) Department of Anatomy, National University of Singapore, Kent Ridge, Singapore |
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Abstract: | Vanadium has recently been reported to induce interphase and M-phase (mitotic) programmed cell death via the generation of hydroxyl free radicals (OH*). In this paper, the effects of antioxidants on: (a) vanadyl[IV]-generated OH* free radical levels; and (b) cellular glutathione in vanadyl [IV]-treated Chang liver cells were evaluated. The surface morphology of vanadyl-treated mitotic cells was studied by confocal and scanning microscopy. The free radical scavengers zinc chloride, glucose and thiourea reduced the levels of vanadyl-induced OH* free radicals and partially prevented the depletion of cellular glutathione. Concurrent with OH* free radical production, vanadyl-treated telophase cells exhibited excessive cell blebbing and cell shrinkage. The morphological features demonstrated in vanadyl-induced mitotic programmed cell death as a consequence of oxidative stress is novel. |
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Keywords: | confocal microscopy glutathione hydroxyl free radicals scanning electron microscopy vanadium |
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