Characteristics and differential T-cell dependency of human B-cell colony precursors |
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Authors: | R Whisler Y Newhouse |
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Affiliation: | Division of Immunology, Department of Medicine, The Ohio State University, Columbus, Ohio 43210 USA |
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Abstract: | Studies were performed to characterize the human peripheral blood non-T cells forming colonies in semisolid cultures stimulated with Staph protein A (SpA). Negative selection experiments revealed that colony precursors largely consisted of cells bearing Fc receptors, complement receptors (CR), surface immunoglobulin (sIg), and Ia-like antigens. Most colony precursors expressed sIgM and sIgD, but not sIgG. Also, colony-forming cells were shown to be distinct from non-T cells proliferating in SpA-stimulated liquid cultures as evidenced by the greater sensitivity of colony precursors to anti-K,λ, or -Ia plus complement depletion. Two distinct categories of colony-forming cells could be distinguished by the expression of CR. CR-positive cells were responsible for greater than 85% of the colonies formed in the absence of optimal T cell numbers. Although under identical conditions CR? cells demonstrated minimal colony growth, the addition of optimal T cell numbers significantly augmented colony responses. Thus, colony precursors express surface markers characteristic of B cells relatively advanced in the developmental pathway. However, less advanced cells are capable of colony growth in the presence of optimal T cell numbers. |
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Keywords: | To whom correspondence should be addressed. |
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