Cytoprotective effects of the antioxidant phytochemical indicaxanthin in beta-thalassemia red blood cells |
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Authors: | Tesoriere L Allegra M Butera D Gentile C Livrea M A |
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Affiliation: | Dipartimento Farmacochimico Tossicologico e Biologico, Università di Palermo, Via Archirafi 32, Palermo 90128, Italy. mal96@unipa.it |
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Abstract: | Antioxidant phytochemicals are investigated as novel treatments for supportive therapy in β-thalassemia. The dietary indicaxanthin was assessed for its protective effects on human β-thalassemic RBCs submitted in vitro to oxidative haemolysis by cumene hydroperoxide. Indicaxanthin at 1.0-10 μM enhanced the resistance to haemolysis dose-dependently. In addition, it prevented lipid and haemoglobin (Hb) oxidation, and retarded vitamin E and GSH depletion. After ex vivo spiking of blood from thalassemia patients with indicaxanthin, the phytochemical was recovered in the soluble cell compartment of the RBCs. A spectrophotometric study showed that indicaxanthin can reduce perferryl-Hb generated in solution from met-Hb and hydrogen peroxide (H2O2), more effectively than either Trolox or vitamin C.
Collectively our results demonstrate that indicaxanthin can be incorporated into the redox machinery of β-thalassemic RBC and defend the cell from oxidation, possibly interfering with perferryl-Hb, a reactive intermediate in the hydroperoxide-dependent Hb degradation. Opportunities of therapeutic interest for β-thalassemia may be considered. |
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Keywords: | Antioxidants betalains haemoglobin indicaxanthin phytochemicals red blood cells |
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