Separation and purification of individual neurofilament proteins by reverse-phase high-performance liquid chromatography |
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| Affiliation: | 1. EMG Unit, Neurology Department, Hospital Clinic of Barcelona, Barcelona, Spain;2. IDIBAPS (Institut d''Investigació Augustí Pi i Sunyer), Facultat de Medicina, University of Barcelona, Barcelona, Spain;3. Dipartimento di Scienze Mediche, Chirurgiche, Neurologiche, Metaboliche e dell''Invecchiamento, Seconda Università degli studi di Napoli, Naples, Italy |
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| Abstract: | A reverse-phase HPLC method was developed to separate individual neurofilament proteins (210,000, 160,000, and 70,000 Da) from the glial fibrillary acid protein. It is useful for analytical or preparative methods, with yields higher than 80%. The method represents improvement over previous methods in speed, efficiency, and purity. Combining this HPLC method with the conventional chromatographic method on DEAE-cellulose, highly purified individual neurofilament proteins can be obtained in large scale. |
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