Effect of dispersing oil phase on the biodegradability of a solid alkane dissolved in non-biodegradable oil |
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Authors: | Hori K Matsuzaki Y Tanji Y Unno H |
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Institution: | (1) Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226–8501, Japan, |
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Abstract: | Acinetobacter sp. CR was grown on a model oil, which consisted of an inert oil matrix of pristane with n-heneicosane dissolved in it as the sole carbon source, in a stirred-tank bioreactor. This bacterium takes up substrates from
the oil phase by direct contact with the oil phase. A previously established mathematical model was applied to reveal the effect of agitation conditions on the growth and n-alkane degradation kinetics of the bacterium. Higher impeller speed resulted in both lower microbial growth and lower n-alkane degradation rate of the bacterium, although it increased the specific surface area of the oil, which was measured
by a previously developed device. This result was due to the decreased number of cells adhering to the oil surface, i.e.,
intense agitation inhibited the adhesion of cells to the oil surface. The addition of a surfactant below a critical micelle
concentration (CMC) inhibited the degradation of n-heneicosane dissolved in pristane, although the biodegradability of the substrate recovered gradually with the increase in
the dose of surfactant over CMC. The results suggest that efforts to increase the specific surface area of the oil phase have
the undesirable result of inhibiting oil degradation when the dominant microbial degraders take up substrates in oil by direct
contact with the oil.
Electronic Publication |
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