Studies with FtsA-LacZ protein fusions reveal FtsA located inner-outer membrane junctions |
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Authors: | Y Chon R Gayda |
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Institution: | Department of Microbiology, Louisiana State University, Baton Rouge 70803. |
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Abstract: | Four FtsA-LacZ translational gene fusions were constructed using a mini-Mu transposon (MudII 1734). FtsA-LacZ fusions and FtsA protein that were radioactively labelled using maxicell technique fractionated identically into membranes and cytoplasm. The FtsA-LacZ fusion proteins were also localized in wild type dividing cells using beta-galactosidase activity. Fractions from a modified sucrose equilibrium gradient exhibited beta-galactosidase activity in fractions corresponding to outer membrane-heavy (OMH) and outer membrane light (OML). The data are consistent with a model in which FtsA protein is incorporated into septal adhesion sites associated with cell division. |
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