Cryopreservation of Leucocytozoon caulleryi sporozoites |
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Authors: | T Moril T Matsui T Iijima N Abe A Yoshizawa M Chikatsune M Watanabe |
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Affiliation: | Department of Parasitology, Kyorin University School of Medicine, Tokyo, Japan. |
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Abstract: | Leucocytozoon caulleryi sporozoites that had been stored at -196 degrees C or -80 degrees C for 6 or 12 months in Eagle's minimum essential medium or Medium 199 supplemented with 5% glycerol and 10% chicken serum showed infectivity to chickens. Glycerol at a concentration of 10% and dimethyl sulfoxide at 10% and 5% were found to be ineffective cryoprotective agents for the low temperature preservation of sporozoites. Sporozoites isolated from the intact females of Culicoides arakawae, which had been stored at -80 degrees C for 6 or 12 months without cryoprotective agents, retained their infectivity. No differences were observed in the prepatent period, duration of parasitemia, and presence of serum-soluble antigens between chickens infected with frozen sporozoites and those infected with fresh sporozoites. |
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