Carbon monoxide and hydroxymercuribenzoate sensitivity of a fatty acid (omega-2) hydroxylase from Bacillus megaterium. |
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Authors: | R S Hare A J Fulco |
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Institution: | University Institute of Microbiology Øster Farimagsgade 2A DK-1353 Copenhagen K, Denmark |
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Abstract: | DNA-free minicells of will not allow growth of phage T-7, nor is RNA synthesis stimulated by phage infection. Thus, these miniature cells seem not to contain RNA polymerase activity. However, DNA-dependent RNA polymerase activity can be unmasked in extracts with poly(dA-T) and Mn2+. This activity may represent a cytoplasmic pool of inactive RNA polymerase in normal cells. |
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