A new interference-free lysine biosensor using a non-conducting polymer film |
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| Authors: | A Curulli S Kelly C O'Sullivan GG Guilbault G Palleschi |
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| Institution: | aCentro C.N.R. di Studio per l'Elettrochimica e la Chimica Fisica delle Interfasi, Via del Castro Laurenziano 7, 00161 Rome, Italy;bUniversity College Cork, Cork, Ireland;cDipartimento di Scienze e Tecnologie Chimiche, Universitaà di Roma Tor Vergata, Rome, Italy |
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| Abstract: | An electrochemical biosensor for the determination of lysine to be used for rapid evaluation of food quality has been developed. Platinum electrodes have been coated by electropolymerisation with 1,2-diaminobenzene (1.2-DAB) using cyclic voltammetry. The reduction in the oxidation of interferents compared with the bare platinum electrode was 100% for ascorbic acid, 99% for acetaminophen and 99% for cysteine. The enzyme L-lysine-α-oxidase was then immobilised onto the polymer layer by passive adsorption and a calibration curve for lysine constructed. This gave a linear range of 1×10−5 mol/l to 1×10−3 mol/l and a limit of detection of 2×10−7 mol/l. |
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| Keywords: | Non-conducting polymers Electropolymerisation Interferences Lysine Food analysis Lysine oxidase |
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