New approaches to the real-time detection of nucleotide mismatches by means of chimeric hybridization probes |
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| Authors: | R. R. Garafutdinov Yu. M. Nikonorov D. A. Chemeris B. N. Postrigan O. V. Chubukova R. F. Talipov V. A. Vakhitov A. V. Chemeris |
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| Affiliation: | 1.Institute of Biochemistry and Genetics, Ufa Scientific Center,Russian Academy of Sciences,Ufa,Russia;2.Bashkir State University,Ufa,Russia |
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| Abstract: | New approaches to the detection of impaired nucleotides based on the allele specific ligation of a “C-probe” followed by rolling circle amplification have been developed. The detection of amplification products was realized by using enzymatic and deoxyribozyme digestion of fluorescently-labeled DNA-RNA-DNA chimeric oligonucleotide structures in cycling probe technology (CPT) in real-time mode. |
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