脂肪酶抑制剂产生菌Bacillus sp.LF深层发酵条件研究

随着生活水平的不断提高 ,肥胖在中国也开始成为影响人们健康的主要危险之一。目前市场上常见的减肥产品主要是作用于大脑 ,通过抑制食欲减少食物的摄入 ,由于这类药可能产生大脑、心血管及神经系统的副作用 ,不宜长期使用。瘦素 (Leptin)是一种肥胖基因表达的多肽激素 ,也是通过食欲中枢发挥作用 ,可以抑制食欲 ,提高代谢率 ,达到减肥的目的。因而另一类减肥药品脂肪酶抑制剂近年来受到国内外重视。脂肪酶抑制剂可直接阻断人体对脂肪的吸收 ,它不需要通过影响中枢神经系统来抑制人体的食欲 ,而是阻止脂肪在胃肠道的吸收。文献报道脂肪酶抑…

Study on Submerged Culture Conditions of Lipase Inhibitor Producing Strain

The optimum medium and fermentations conditions of the lipase inhibitor producing strain Bacillus sp. LF were studied. The optimal medium was selected after single factor test and orthogonal test as follows: lactose 0.8%, peptone 1%, (NH4)2SO4 0.8%, NaCl 0.5%, original pH 7.2. With 4% (V/V) inoculum in 40 mL medium in 250 mL flask, Bacillus sp. LF could reach high yield of lipase inhibitor after 24 h at 30 degrees C. The highest lipase inhibitory activity in broth could reach 52.3%.