Effects of exercise training and diabetes on cardiac myosin heavy chain composition |
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| Authors: | Dennis J Paulson Mahesh Gupta Radovan Zak Jiansheng Zhao |
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| Institution: | (1) Department of Physiology, Chicago College of Osteopathic Medicine, 60515 Downers Grove, IL, USA;(2) Department of Medicine, University of Chicago, 60637 Chicago, IL, USA;(3) Department of Physiology, Chicago College of Osteopathic Medicine, 555 31st Street, 60515 Downers Grove, IL, USA |
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| Abstract: | This study determined whether the beneficial effects of exercise training on the diabetic heart previously observed are associated with alterations in ventricular myosin heavy chain (MHC) isoform composition. Diabetes was induced in rats by i.v. streptozotocin. Trained rats were run on a treadmill for 60 min/day, 27 m/min, 10% grade. After 10 wks, ventricular MHC isoenzyme protein composition was analyzed for MHC composition using gel electrophoresis.  -MHC and  -MHC mRNA were determined by Northern and slot blot hybridization techniques. Both protein and mRNA analyses indicated that sedentary control rats exhibited a predominance of -MHC. Sedentary diabetics exhibited a shift to -MHC. Exercise trained diabetic rats showed a predominance of -MHC. The results indicate that treadmill exercise training of diabetic rat does not prevent the diabetes-induced shift in MHC composition towards the -MHC isoform, thus it is unlikely that the beneficial effects of exercise training on the diabetic heart, previously shown, are due to a normalization of the myosin isoform composition. |
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| Keywords: | diabetes diabetic cardiomyopathy myosin messenger RNA |
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