| 种子特异性启动子（napinB promoter）分离，表达载体构建及转基因植物获得 |
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| 引用本文： | 张景昱,宋艳茹,李丽,杜桂森. 种子特异性启动子（napinB promoter）分离，表达载体构建及转基因植物获得[J]. 植物学通报, 2001, 18(2): 216-220 |
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| 作者姓名： | 张景昱 宋艳茹 李丽 杜桂森 |
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| 作者单位： | 1. 中国科学院植物研究所
2. 首都师范大学生物系 |
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| 摘 要： | 利用PCR技术从油菜Brassica napus H165基因组DNA中分离了napinB启动子，序列分析表明，扩增片段（nap300)与文献报道的napinB启动子相应区域的同源性为97％，将其与gus连接构建种子特异性表达载体，农杆菌介导转化烟草，PCR，Southern结果显示，nap300已整合到烟草基因组DNA，获得了转基因植株。
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| 关 键 词： | 种子 特异性启动子 植物 表达载体 转基因植株 |
| 修稿时间： | 2001-01-16 |
Isolation of Seed SpecificPromoter (napinB promoter), Construction of Expression Vector and Obtainment of TransgenicTobacco Plants |
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| LI Li,ZHANG Jing-Yu,SONG Yan-Ru,DU Gui-Sen. Isolation of Seed SpecificPromoter (napinB promoter), Construction of Expression Vector and Obtainment of TransgenicTobacco Plants[J]. Chinese Bulletin of Botany, 2001, 18(2): 216-220 |
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| Authors: | LI Li ZHANG Jing-Yu SONG Yan-Ru DU Gui-Sen |
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| Abstract: | A fragment of 300bp (nap300) in .napinB. promter region was isolated by PCR from genomic DNA of .Brassica napus. H165. Nap300 and the corresponding sequence of .napinB. promoter shared 97% homology. Expression vector harboring the promoter fused with GUS gene was constructed for functional analysis. Tobacco plants were transformed by .Agrobacterium tumefaciens._mediated method. PCR and Southern results showed that nap300 had been integrated into genomic DNA of tobacco successfully. Assay of GUS activity in the seeds of transgenic tobacco will be carried out to identify the function of the promoter. |
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| Keywords: | Seed_specific promoter Plant expression vector GUS Transgene Tobacco |
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