Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51 |
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| Authors: | Ochiai Kazuhiko Yoshikawa Yasunaga Yoshimatsu Kumiko Oonuma Toshina Tomioka Yukiko Takeda Eichi Arikawa Jiro Mominoki Katsumi Omi Toshinori Hashizume Kazuyoshi Morimatsu Masami |
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| Institution: | Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan. |
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| Abstract: | The breast cancer susceptibility protein BRCA2 is essential for recombinational DNA repair. BRCA2 specifically binds to RAD51 via eight BRC repeat motifs and delivers RAD51 to double-stranded DNA breaks. In this study, a mammalian two-hybrid assay and competitive ELISA showed that the interaction between BRC repeat 4 (BRC4) and RAD51 was strengthened by the substitution of a single BRC4 amino acid from valine to isoleucine (V1532I). However, the cancer-associated V1532F mutant exhibited very weak interaction with RAD51. This study used a comparative analysis of BRC4 between animal species to identify V1532 as an important residue that interacts with RAD51. |
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