Cytoskeletal association of pp60 : The transforming protein of the rous sarcoma virus

Immunoferritin labelling methods have been employed to examine the distribution of the Rous Sarcoma virus (RSV)-transforming protein pp60^src in the detergent-resistant cytoskeleton of transformed cells. pp60^src was found to be localized on actin microfilaments present in adhesion plaques, at adherens junctions between cells and also in microfilament bundles. This localization is consistent with the hypothesis that some of the morphological effects of transformation result from the interaction in situ of pp60^src with microfilament-bound target proteins.