| 云南美味牛肝菌ITS区域结构特点 |
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| 引用本文： | 赵永昌,方琳,张荀,左文昊,李树红,李姌,柴红梅,钟明惠.云南美味牛肝菌ITS区域结构特点[J].云南植物研究,2006,28(6):575-580. |
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| 作者姓名： | 赵永昌 方琳 张荀 左文昊 李树红 李姌 柴红梅 钟明惠 |
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| 作者单位： | 云南省农业科学院生物技术与种质资源研究所,云南,昆明,650223 |
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| 摘 要： | 利用ITS的通用引物（ITS5-ITS4）对云南的美味牛肝菌（Boletus edulis）子实体的DNA进行PCR扩增，扩增产物回收后直接测序。序列的聚类分析表明，在ITS1—5．8S rDNA-ITS2区域，云南的美味牛肝菌与欧洲的夏牛肝菌（B．aestivalis）和铜色牛肝菌（B．aereus）同源性较高，但在ITS2区域夏牛肝菌和铜色牛肝菌分别有一段美味牛肝菌没有的大小分别为73bp和26bp的特征序列。
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| 关 键 词： | 美味牛肝菌 同源性 |
| 文章编号： | 0253-2700（2006）06-575-06 |
| 收稿时间： | 2006-02-24 |
| 修稿时间： | 2006-07-10 |
The Structural Characteristics of ITS Region of Boletus edulis ( Boletoueae ) in Yunnan |
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| ZHAO Yong-Chang,FANG Lin,ZHANG Xun,ZUO Wen-Hao,LI Shu-Hong,LI Ran,CHAI Hong-Mei,ZHONG Ming-Hui.The Structural Characteristics of ITS Region of Boletus edulis ( Boletoueae ) in Yunnan[J].Acta Botanica Yunnanica,2006,28(6):575-580. |
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| Authors: | ZHAO Yong-Chang FANG Lin ZHANG Xun ZUO Wen-Hao LI Shu-Hong LI Ran CHAI Hong-Mei ZHONG Ming-Hui |
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| Institution: | Institute of Biotechnology and Germplasm Resource, Yunnon Academy of Agricultural Science, Kunming 650223, China |
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| Abstract: | A pair of general primer (ITS5-ITS4) was used to test in amplification of internal transcribed spacer (ITS) region of chromosomal DNA of fruiting bodies of Boletus edulis in Yunnan.The DNA amplification fragment was purified with DNA Gel Extraction Kit and sequenced by CEQ8000.The result of phylogenetic analyses based on ITS1-5.8S-ITS2 sequences showed that B.edulis in Yunnan had high homology with B.aestivalis and B.aereus in Europe.73?bp and 26?bp DNA characteristic fragments differing from all B.edulis were found in the ITS-region of B.aereus and B.aestivalis respectively. |
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| Keywords: | PCR |
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