cDNA cloning of a mandibular organ inhibiting hormone from the spider crabLibinia emarginata |
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Authors: | Lei Liu Hans Laufer Peter J Gogarten Minhua Wang |
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Institution: | (1) Department of Molecular and Cell Biology, University of Connecticut, 06268 Storrs, CT, USA;(2) Present address: Department of Computer Science and Engineering, University of Connecticut, 06268 Storrs, CT, USA |
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Abstract: | Mandibular organs (MO) produce a crustacean juvenile hormone, methyl farnesoate (MF). MO activity is negatively regulated
by factors, called mandibular organ inhibiting hormones (MOIHs), from the crustacean sinus gland X-organ complex in the eyestalks.
Three MOIHs have been isolated previously from the spider crabLibinia emarginata and are characterized as members of the crustacean hyperglycemic hormone (CHH) neuropeptide family. In the research reported
here, a full length cDNA sequence of 972 bp of a MOIH was isolated by screening a cDNA library constructed from the eyestalks
ofLibinia emarginata. This cDNA sequence encodes a preprohormone peptide with 137 amino acid residues, including a 26-amino acid long signal peptide,
a 34-amino acid long precursor peptide, a dibasic peptide, the full length of 72-amino acid long MOIH, and a tri-peptide Gly-Lys-Lys
which designates the potential amidation site at the C-terminus of the mature peptide. |
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Keywords: | crustacea MOIH mandibular organ cDNA cloning sinus gland neuropeptide |
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