Plasmids supplying the Q-qut-controlled gam function permit growth of lambda red- gam- (Fec-) bacteriophages on recA- hosts |
| |
| Authors: | G F Crouse |
| |
| Institution: | 1. Department of Biology, Emory University, Atlanta, GA 30322 U.S.A. Tel. (404) 727-4234;2. Laboratory of Molecular Biology, LBI — Basic Research Program, National Cancer Institute, Frederick Cancer Research Facility, Frederick, MD 21701 U.S.A. |
| |
| Abstract: | A plasmid, pgam, has been constructed which expresses the phage lambda gene, gam, under the control of the lambda late promoter, p'R, contained in a form of a p'R-qut-t'R1 module. Lambda red- gam-, which normally do not grow on recA- hosts, are able to grow on recA- hosts containing pgam, because their Q function can turn on the gam gene expression. This facilitates cloning with lambda red- gam- vectors in recA- hosts. |
| |
| Keywords: | Recombinant DNA plasmid cloning systems replication Ap ampicillin bp base pair(s) kb 1000 bp LB Luria broth moi multiplicity of infection pfu plaque-forming units Q-utilization site (Somasekhar and Szybalski 1983) resistance sensitivity Sm streptomycin Spi [] designates plasmid-carrier state |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
