Chlorophyll Formation in Euglena gracilis var. bacillaris: Effect of Chelating Agents |
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Authors: | P. J. DUBASH D. V. REGE |
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Affiliation: | Dept. of Chemical Technology, Univ. of Bombay, Matunga, Bombay-19, India |
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Abstract: | SYNOPSIS. 8-Hydroxyquinoline (8-HQ) and cysteine markedly inhibit the synthesis of chlorophylls in non-proliferating etiolated Euglena gracilis var. bacillaris on illumination. This is thought to be due, at least to some extent, to the binding of Mg2+ ions in the non-proliferation medium, but largely as a consequence of intracellular chelating action. The effect of 8-HQ could be reversed by the presence of metal ions higher in the Mellor-Malley series than Mg2+, e.g., Zn2+ and Co2+. Sodium diethyldithiocarbamate (Na-DDG) and ethylenediaminetetraacetic acid (EDTA) have no effect on chlorophyll synthesis in non-proliferation conditions. Growth in the light is more affected than growth in the dark by all these chelating agents, including Na-DDG and EDTA. This is due to the differential requirement of Mg2+ under these 2 growth conditions. |
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