Protein kinase C-gamma is present in adriamycin resistant HL-60 leukemia cells |
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| Authors: | A Aquino B S Warren J Omichinski K D Hartman R I Glazer |
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| Institution: | Laboratory of Biological Chemistry, National Cancer Institute, Bethesda, Maryland 20892. |
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| Abstract: | The isoform pattern of protein kinase C (PKC) was examined in wild-type and Adriamycin-resistant (HL-60/AR) HL-60 leukemia cells. Analyses were carried out by immunoblotting with mouse monoclonal antibodies against PKC-alpha and PKC-beta and a rabbit polyclonal antibody against the variable (V3) region of PKC-gamma. HL-60/AR cells contained an equivalent level of PKC-alpha and a lower amount of PKC-beta than HL-60 cells. In contrast, only HL-60/AR cells contained PKC-gamma. These results indicate that the regulation of this family of isoenzymes is altered in drug-resistant cells. |
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