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黑龙江林蛙不同部位中氨基酸的含量分析
引用本文:曹阳,张若文,陈晓辉,付春旺,毕开顺.黑龙江林蛙不同部位中氨基酸的含量分析[J].氨基酸和生物资源,2010,32(2):17-19.
作者姓名:曹阳  张若文  陈晓辉  付春旺  毕开顺
作者单位:沈阳药科大学,药学院,辽宁沈阳,110016;贝尔法斯特女王大学,药学院,McClay 研究中心,英国 BT97BL;沈阳药科大学,中药学院,辽宁沈阳,110016
摘    要:采用茚三酮柱后衍生-氨基酸分析法,对黑龙江林蛙油、卵、皮中17种氨基酸的组成和含量进行测定。结果显示黑龙江林蛙油、卵、皮均含有17种氨基酸,氨基酸总量分别为356.5 mg.g-1,525.1 mg.g-1,752.5 mg.g-1,其中必需氨基酸分别占氨基酸总量的46.06%,38.89%,33.33%。通过对黑龙江林蛙油、卵、皮中氨基酸含量的对比分析,表明林蛙油中氨基酸的营养价值最高。

关 键 词:黑龙江林蛙  氨基酸分析仪  氨基酸  林蛙油

Determination of Amino Acids in Different Parts of Rana amurensis Boulenger
CAO Yang,ZHANG Ruo-wen,CHEN Xiao-hui,FU Chun-wang,BI Kai-shun.Determination of Amino Acids in Different Parts of Rana amurensis Boulenger[J].Amino Acids & Biotic Resources,2010,32(2):17-19.
Authors:CAO Yang  ZHANG Ruo-wen  CHEN Xiao-hui  FU Chun-wang  BI Kai-shun
Institution:CAO Yang1,ZHANG Ruo-wen3,CHEN Xiao-hui1,FU Chun-wang2,BI Kai-shun1(1.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016;2.School of Traditional Chinesne Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China;3.McClay Research Centre,School of Pharmacy,Queen’s University Belfast,BT97BL,England)
Abstract:The contents and the ingredints of the amino acids in Rana amurensis Boulenger were determined with Hitachi 2622 cation exchange chromatographic column(4.6 mm×60 mm) by post-column Ninhydrin derivatization-amino acid analyzer method.There were 17 kinds of amino acids in oviduct ranae,egg,skin and their total amino acid contents were 356.5 mg·g-1,525.1 mg·g-1,752.5 mg·g-1,respectively.The essential amino acid contents in their total amino acid contents were 46.06%,38.89%,33.33%,respectively.The nutritious value of oviduct ranae was the highest in Rana amurensis Boulenger.
Keywords:Rana amurensis Boulenger  amino acid analyzer  amino acid  oviduct ranae
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