Restoration of Ca2+-activated tension of CDTA-treated single skeletal muscle fibers by troponin C |
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Authors: | S Morimoto T Fujiwara I Ohtsuki |
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Institution: | Department of Pharmacology, Faculty of Medicine, Kyushu University, Fukuoka. |
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Abstract: | Single fibers from glycerinated rabbit psoas muscle were treated with a solution containing CDTA, a strong chelator of metal ions. The CDTA-treated fibers lost all of the troponin C and showed no Ca2+-activated tension development. The addition of troponin C restored the Ca2+-activated tension of CDTA-treated fibers. The tension-pCa relationship in the case of the CDTA-treated fibers reconstituted with troponin C was almost the same as that in the case of the same fibers before the CDTA treatment. These results are consistent with those of the previous study on the Ca2+-activated ATPase of CDTA-treated rabbit skeletal myofibrils. |
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