Cinematographic analysis of vascular smooth muscle cell interactions with extracellular matrix |
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Authors: | Marlene Absher Linda Baldor |
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Affiliation: | (1) Department of Medicine, University of Vermont, 05405 Burlington, Vermont;(2) Department of Physiology & Biophysics, University of Vermont, 05405 Burlington, Vermont |
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Abstract: | Summary The interactions of vascular smooth muscle cells with growth modulators and extracellular matrix molecules may play a role in the proliferation and migration of these cells after vascular injury and during the development of atherosclerosis. Time-lapse cinematographic techniques have been used to study cell division and migration of bovine carotid artery smooth muscle cells in response to matrix molecules consisting of solubilized basement membrane (Matrigel) and type I collagen. When cells were grown adjacent to Matrigel, both migration and cell proliferation were increased and interdivision time was shortened. Cells grown in Matrigel or in type I collagen had markedly reduced migration rates but interdivision time was not altered. Further, diffusible components of the Matrigel were found to stimulate proliferation of the smooth muscle cells. This work was supported by grants HL35684 and SCOR HL14212 from the National Institutes of Health, Bethesda, MD. |
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Keywords: | smooth muscle cells cell migration cell division time extracellular matrix cinematography |
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