Heterogeneity of chicken photoreceptors as defined by hybridoma supernatants |
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| Authors: | Dr Á Szél L Takács É Monostori I Vigh-Teichmann P Röhlich |
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| Institution: | (1) Second Department of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Budapest;(2) Laboratory I of Electron Microscopy, Semmelweis University of Medicine, Budapest;(3) Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary;(4) Second Dept. of Anatomy, Histology and Embryology, Semmelweis University of Medicine, H-1450 Budapest, Tüzoltó u. 58, Hungary |
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| Abstract: | Summary Immune cells producing antibodies to chicken photoreceptor membranes were fused with myeloma cells and supernatants of the resulting hybridoma cells were used to test various types of photoreceptor cells in the chicken retina by means of immunocytochemistry. A polyclonal antibody raised against the protein component of bovine rhodopsin was also used.Outer segments of various photoreceptor cells were labelled by the following antibodies: rods were positive with the anti-rhodopsin antibody, both members of the double cones were stained by supernatant A1, while one type of single cones (designated as type A) was specifically labelled by supernatants A5, B3 and D6. The other type of single cones (type B) reacted with anti-rhodopsin and supernatant A1. The results indicate that there are distinct differences in the molecular structure of various photoreceptor outer segments. |
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| Keywords: | Retina chicken Photoreceptor outer segments Photopigments Immunological heterogeneity Antibodies Immunocytochemistry |
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