Nitrogen-source-induced activation of neutral trehalase in Schizosaccharomyces pombe and Pachysolen tannophilus: role of cAMP as second messenger |
| |
Authors: | T Soto J Fernández J Vicente-Soler J Cansado M Gacto |
| |
Institution: | Department of Genetics and Microbiology. Facultad de Biologia, University of Murcia, 30071 Murcia, Spain |
| |
Abstract: | Abstract Resting cells of the fission yeast Schizosaccharomyces pombe , suspended in buffer with glucose, responded to the addition of asparagine by increasing trehalase activity. This response was preceded by a peak in cAMP concentration. The addition of the nitrogen source to resting cells, devoid of the catalytic subunit of cAMP-dependent protein kinase, produced the transient increase in cAMP but did not promote any change in trehalase activity. In the budding yeast Pachysolen iannophilus , the activation of trehalase by nitrogen source was also accompanied by a sharp peak in cAMP. These results suggest that in the two yeasts cAMP acts as a second messenger in the transduction of the nitrogen-source-induced signal causing the activation of trehalase. |
| |
Keywords: | Trehalase activation cAMP Signal transduction Schizosaccharomyces pombe Pachysolen tannophilus |
|
|