Roles of metal ions in nucleases

The hydrolysis of phosphodiester bonds by metallonucleases is crucial to most aspects of nucleic acid processing. In recent years, studies of the classical restriction endonucleases have given way to the characterization of metallonucleases with widely divergent active site motifs. These developments fuel debates regarding the roles of metal ions in these enzymes. It is fortuitous that the current literature also includes the increased application of a variety of computational techniques to test the roles of metal ions in nucleic acid hydrolysis by these systems. This includes recent proposals and indirect evidence that these enzymes utilize metal ion movement in these reactions.