Marine lysozyme from a marine bacterium that inhibits angiogenesis and tumor growth |
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Authors: | Junli Ye Chunbo Wang Xuehong Chen Shenbo Guo Mi Sun |
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Institution: | (1) Medical College, Qingdao University, 422 Room, Boya Building, 308 Ningxia Road, Qingdao, 266071, People’s Republic of China;(2) Open laboratory of Marine Enzyme and Enzyme Engineering, Yellow Sea Fisheries Research Institute, Qingdao, 266071, People’s Republic of China |
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Abstract: | Recent studies suggest that lysozyme, rich in hen egg, has an antitumor function. In the present study, we investigated the
antitumor and antiangiogenesis effects of a newly isolated marine lysozyme both in vitro and in vivo. First, we showed that
this marine-derived lysozyme specifically inhibits the proliferation of endothelial cells (ECV304) in a dose-dependent manner
with no cytotoxicity (IC50 = 3.64 μM). Second, we showed that this marine lysozyme directly suppresses neovascularization in chicken embryos using chorioallantoic
membrane assay. Third, we demonstrated that this marine lysozyme markedly inhibits tumor growth in mice bearing either sarcoma
180 or hepatoma 22. Unexpectedly, hen egg lysozyme has no effects on the proliferation of endothelial cells in vitro or neovascularization
in chicken embryos or tumor growth in nude mice at the same dosage range. Taken together, our studies clearly show that the
newly identified marine lysozyme is a potent antitumor molecule, which may inhibit tumor growth and inhibit angiogenesis.
We believe that this marine lysozyme may have a therapeutic value in antitumor drug development. |
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Keywords: | Lysozyme Antiangiogenesis Endothelial cells CAM assay Antitumor Marine lysozyme |
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