Lipid content of human platelets quantitated by thin-layer chromatography in combination with flame ionization detection |
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Authors: | Kazuko Hiramatsu Hiroyuki Nozaki Shigeru Arimori |
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Institution: | The Fourth Department of Internal Medicine School of Medicine, Tokai University, Isehara, 259-11 Japan |
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Abstract: | The lipid contents of human platelets from twenty-one healthy adults were analysed using thin-layer chromatography in combination with flame ionization detection.The weight per cent of neutral lipids in human platelets was 14.6%, which consisted mainly of free cholesterol, that of phosphatidylethanolamine 24.9%, phosphatidylserine plus phosphatidylinositol 6.8%, phosphatidylcholine 35.2% and sphingomyelin 18.6%. Free cholesterol in 108 platelets was estimated as 7 μg and phospholipids as 46 μg from calibration standards. The reproducibility was satisfactory and the procedure could be performed quickly and simply. |
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