cDNA cloning of artichoke mottled crinkle virus RNA and localization and sequencing of the coat protein gene |
| |
Authors: | Mario Tavazza Alessandra Lucioli Giorgio Ancora Eugenio Benvenuto |
| |
Affiliation: | (1) Dipartimento Agrobiotecnologie, Divisione Ingegneria Genetica, ENEA, C.P. 2400, Roma A.D., Italy |
| |
Abstract: | We report the cDNA cloning of the genomic RNA of artichoke mottled crinkle virus (AMCV), which is a member of Tombusvirus group. AMCV has a monopartite positive sense RNA genome, which is not polyadenylated at the 3 end. The genome size is 4.8 kb.We have localized and sequenced the open reading frame (ORF) encoding the coat protein. Unlike most monopartite positive-strand RNA plant viruses, the ORF is not located near the 3 end, but like other members of the Tombusvirus group, CyRSV (cymbidium ringspot virus), TBSV-cherry (tomato bushy stunt virus cherry strain) and CNV (cucumber necrosis virus) it starts ca. 2.7 kb downstream of the 5 end and stops ca. 1 kb upstream of the 3 end. This ORF predicts a polypeptide chain of 387 amino acids.Comparison of the coat proteins of AMCV, TBSV-BS3, TBSV-cherry and CNV confirms that, within the Tombusvirus group, there exists a high degree of similarity among coat proteins but that this similarity is not uniformly distributed among domains. In particular, the N-terminal region, thought to make contact with the phosphate groups of the viral RNA, and the C-terminal region, considered the most immunogenic portion of the capsid, are found to be the least homologous. |
| |
Keywords: | artichoke mottled crinkle virus cDNA cloning coat protein protein sequence comparison Tombusvirus |
本文献已被 SpringerLink 等数据库收录! |
|