Integration of Retrotransposons-Based Markers in a Linkage Map of Barley |
| |
Authors: | Monica Rodriguez Donal O’Sullivan Paolo Donini Roberto Papa Elena Chiapparino Fiona Leigh Giovanna Attene |
| |
Institution: | (1) Dipartimento di Scienze Agronomiche e Genetica Vegetale Agraria, Università degli Studi di Sassrai, Via E. De Nicola, 07100 Sassari, Italy;(2) Molecular Research Group, NIAB, Huntingdon Road, Cambridge, CB3 0LE, UK;(3) Dipartimento di Scienze degli Alimenti, Università Politecnica delle Marche, Via Brecce Bianche, 60131, Ancona, Italy |
| |
Abstract: | A deeper understanding of random markers is important if they are to be employed for a range of objectives. The sequence specific
amplified polymorphism (S-SAP) technique is a powerful genetic analysis tool which exploits the high copy number of retrotransposon
long terminal repeats (LTRs) in the plant genome. The distribution and inheritance of S-SAP bands in the barley genome was
studied using the Steptoe × Morex (S × M) double haploid (DH) population. Six S-SAP primer combinations generated 98 polymorphic
bands, and map positions were assigned to all but one band. Eight putative co-dominant loci were detected, representing 16
of the mapped markers. Thus at least 81 of the mapped S-SAP loci were dominant. The markers were distributed along all of
the seven chromosomes and a tendency to cluster was observed. The distribution of S-SAP markers over the barley genome concurred
with the knowledge of the high copy number of retrotransposons in plants. This experiment has demonstrated the potential for
the S-SAP technique to be applied in a range of analyses such as genetic fingerprinting, marker assisted breeding, biodiversity
assessment and phylogenetic analyses. |
| |
Keywords: | Barley Molecular mapping Retrotransposons S-SAP (Sequence-Specific Amplified Polymorphism) markers |
本文献已被 SpringerLink 等数据库收录! |
|