A monochromosomal hybrid cell assay for evaluating the genotoxicity of environmental chemicals |
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| Authors: | Shahbeg S Sandhu Rama D Gudi Raghbir S Athwal |
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| Institution: | (1) Genetic Toxicology Division Health effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina;(2) Department of Microbiology and Molecular Genetics New Jersey Medical School, University of Medicine and Dentistry, Newark, New Jersey;(3) Genetic Toxicology Division, U.S. Environmental Protection Agency, 27711 Research Triangle Park, NC |
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| Abstract: | The development and utilization of a monochromosomal hybrid cell assay for detecting aneuploidy and chromosomal aberrations are described. The monochromosomal hybrid cell lines were produced by a two-step process involving transfer of a marker bacterial gene to a human chromosome and then by integration of that human chromosome into a mouse complement of chromosomes through microcell fusion. For chemically induced aneuploidy, the segregation of a single human chromosome among mouse chromosomes is used as a cytogenetic marker. The genetic assay for aneuploidy is based on the ability of the cells to grow in a medium that selects for the loss of the human chromosome. The assay for clastogenicity is based on survival of the cells after treatment with the chemicals in medium that selects for retention of the human chromosome but loss of its segment containing diphtheria toxin locus. The assays greatly simplify the detection of chromosomal aberrations induced by environmental factors at low-dose levels. |
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| Keywords: | aneuploidy clastogenicity monochromosomal hybrid cells |
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