Protein synthesizing systems from spores and vegetative cells of Bacillus cereus |
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| Authors: | H. A. Douthit R. A. Kieras R. A. Preston |
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| Affiliation: | (1) Department of Cell and Molecular Biology, Division of Biological Sciences, University of Michigan, 48109 Ann Arbor, Michigan, USA;(2) Present address: Department of Cellular and Developmental Biology, Biological Sciences West, University of Arizona, 85721 Tucson, Arizona, USA |
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| Abstract: | A system of polyphenylalanine synthesis was optimized for a comparison of the polymerizing activities of ribosomes from spores and vegetative cells of Bacillus cereus T. Ribosomes of both types react similarly, showing a magnesium optimum of about 6 mM and spermidine optima of about 5 mM and 4 mM for vegetative and spore ribosomes, respectively. These lead to optimum mono- to multivalent cation rations of 9 and 10 respectively at 100 mM ammonium ion. A comparison of the response of these ribosomes to suboptimal concentrations of magnesium and spermidine show that they differ qualitatively from each other, suggesting that they possess different structure, macromolecular or ionic components.Abbreviations DFP diisopropylfluorophosphate - HEPES N-2-hydroxyethylpiperazine-N -ethanesulfonic acid |
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| Keywords: | Ribosomes Spores Bacillus Magnesium Spermidine Protein Synthesis |
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