Strain improvement of Trichoderma reesei Rut C-30 for increased cellulase production |
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Authors: | He Jun Yu Bing Zhang Keying Ding Xuemei Chen Daiwen |
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Affiliation: | (1) Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan, 625 014, P. R. China;(2) Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Yaan, Sichuan, China |
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Abstract: | The strain of Trichoderma reesei Rut C-30 was subjected to mutation after treatment with N-methyl-N′-nitro-N-nitrosoguanidine (NG) for 6 h followed by UV irradiation for 15 min. Successive mutants showed enhanced cellulase production, clear hydrolysis zone and rapid growth on Avicel-containing plate. Particularly, the mutant NU-6 showed approximately two-fold increases in activity of both FPA and CMCase in shake flask culture when grown on basal medium containing peptone (1%) and wheat bran (1%). The enzyme production was further optimized using eight different media. When a mixture of lactose and yeast cream was used as cellulase inducer, the mutant NU-6 yielded the highest enzyme and cell production with a FPase activity of 6.2 U ml−1, a CMCase activity of 54.2 U ml−1, a β-glucosidase activity of 0.39 U ml−1, and a fungal biomass of 12.6 mg ml−1. It deserved noting that the mutant NU-6 also secreted large amounts of xylanases (291.3 U ml−1). These results suggested that NU-6 should be an attractive producer for both cellulose and xylanase production. |
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Keywords: | Cellulase Trichoderma reesei NTG and ultraviolet mutation Fermentation |
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