Sometimes faster can be better: Microneedling IPG strips enables higher throughput for integrative top-down proteomics |
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| Authors: | Katrina Carbonara Jens R Coorssen |
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| Institution: | 1. Department of Health Sciences, Faculty of Applied Health Sciences, Brock University, St. 2. Catharines, Ontario, Canada;3. Department of Biological Sciences, Faculty of Mathematics and Science, Brock University, St. |
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| Abstract: | Passive rehydration of immobilized pH gradient (IPG) strips for two-dimensional gel electrophoresis (2DE) has, to our knowledge, never been quantitatively evaluated to determine an ideal rehydration time. Seeking to increase throughput without sacrificing analytical rigor, we report that a substantially shorter rehydration time is accomplished when surface area of IPG strips is increased via microneedling. Rehydration for 4 h, post microneedling, provides comparable results to overnight rehydration in final analyses by 2DE, while also shortening the overall protocol by 1 day. |
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| Keywords: | proteoforms proteomes rehydration two-dimensional gel electrophoresis |
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