Optimization of Nutritional Factors for Exopolysaccharide Production by Submerged Cultivation of the Medicinal Mushroom Oudemansiella radicata |
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Authors: | Xiang Zou |
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Institution: | (1) School of Life Science, Southwest China Normal University, 2 Tian Sheng Road, Chongqing, Beibei, 400715, P.R. China |
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Abstract: | Summary Effects of nutritional factors on exopolysaccharide production by submerged cultivation of the medicinal mushroom Oudemansiella radicata were investigated in shake flasks. Sucrose and peptone were optimal carbon and nitrogen sources for cell growth and exopolysaccharide
production. The exopolysaccharide production was increased with an increase in initial sucrose concentration within the range
of 10–40 g l−1 and initial peptone concentration within the range of 1–3 g l−1. To enhance further exopolysaccharide production, the effect of carbon/nitrogen ratios was studied using central composite
design (CCD) and response surface analysis. The maximum exopolysaccharide production of 2.67 ± 0.15 g l−1 was achieved in medium with optimized carbon and nitrogen sources, i.e. 39.3 g sucrose l−1 and 3.16 g peptone l−1 in the same cultivation conditions. The information obtained is helpful for the hyperproduction of exopolysaccharide by submerged
cultivation of O. radicata on a large scale. |
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Keywords: | Central composite design exopolysaccharide medicinal mushroom Oudemansiella radicata submerged cultivation |
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