Expression ofBacillus subtilis endoglucanase gene inLactobacillus acidophilus |
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Authors: | Byung-Hak Baik M. Y. Pack |
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Affiliation: | (1) Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Cheongryang, P. O. Box 150, Seoul, Korea |
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Abstract: | Summary ABacillussubtilis gene which codes for endoglucanase was transferred intoLactobacillusacidophilus by electroporation with plasmids containing the gene. The endoglucanase gene expressed well in the transformed cells and most of the gene product was found in the culture medium. The efficiency of the endoglucanase production by the transformed.L.acidophilus cells depended greatly on the choice of the vector plasmids on which the gene to be inserted. |
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