The use of nitrosoguanidine in the study of mutagenesis in a non-acid-fast strain ofMycobacterium phlei |
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Authors: | J Koní?ek I Málek |
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Institution: | (1) Department of Bacterial Genetics, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 4 |
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Abstract: | N-methyl-N-nitroso-N′-nitroguanidine was used for the induction of two types of mutations in the PN strain ofMycobacterium phlei. Nineteen auxotrophic, 136 scotochromogenic and 50 achromogenic mutants were isolated after of treatment with nitrosoguanidine
at a concentration of 1,000 μg/ml. Auxotrophic mutants required primarily amino acids and vitamins and half of them may be
used for further genetic work due to their low frequency of spontaneous reversions. Colonies of scotochromogenic mutants were
orange with the exception of one, which was strawberry red. Most scotochromogenic mutants were detected on a streptomycin
containing medium. Roughly two thirds of the scotochromogenic mutants and one half of achromogenic mutants did not revert
to the original photochromogenic character. |
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