Determination of niacin metabolites 1-methyl-5-carboxylamide-2-pyridone and n-1-methylnicotinamide in urine by high-performance liquid chromatography |
| |
Authors: | R. C. Terry M. Simon |
| |
Affiliation: | Research Department, Bio-Science Laboratories, Van Nuys, CA 91405 U.S.A. |
| |
Abstract: | A test suitable for detecting latent niacin deficiency was developed. It measures the 24-h urinary output of the two major metabolites of niacin, 1-methyl-5-carboxylamide-2-pyridone and N-1-methylnicotinamide. The two metabolites were isolated from urine using separate ion-exchange extractions. They and their two internal standards were quantitated simultaneously by high-performance liquid chromatography using a reversed-phase ion pairing separation. Detection was by absorbance at 254 nm. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|