高寒湿地土壤微生物区系组成对氮添加的响应

土壤微生物是土壤养分循环的关键驱动者,对土壤环境变化感应明显,氮是陆地生态系统的限制元素之一,其改变可能会给生态系统物种多样性造成一定影响.为了解高寒湿地土壤微生物组成对氮添加的响应,以青海湖流域高寒湿地为研究对象,通过(0 g·m-2、0.5 g·m-2、1 g·m-2、1.5 g·m-2、2 g·m-2、2.5 g...

Response of microbial flora to nitrogen addition in alpine wetlands

Soil microorganisms are the key drivers of soil nutrient cycle and have obvious induction to soil environmental change.Nitrogen is one of the limiting elements of terrestrial ecosystem,and its change may have a certain impact on the species diversity of the ecosystem.In order to understand the response of soil microbial composition to nitrogen addition in alpine wetland of Qinghai Lake Basin,eight levels of simulated nitrogen addition tests were conducted(0 g·m^-2,0.5 g·m^-2,1 g·m^-2,1.5 g·m^-2,2 g·m^-2,2.5 g·m^-2,5 g·m^-2,10 g·m^-2).The results showed that nitrogen addition reduced the Chao1 index of bacterial fungi and archaea,and the Shannon index of bacteria and archaea increased significantly(P<0.05).The Shannon index of fungi decreased significantly(P<0.05).The nitrogen deposition changed the microbial community structure,and the relative abundance of Proteobacteria,Actinobacteria,Euryarchaeota and Crenarchaeota decreased,while that of Acidobacteria and Ascomycota increased.Fungal communities have a low response to nitrogen addition and may be more adaptive to the environment than bacterial and archaea communities.These results indicate that the effects of nitrogen addition on different soil microorganisms are different.Nitrogen deposition is a process of continuous accumulation,and long-term nitrogen deposition is bound to cause changes in soil microbial community and affect the stability of the ecosystem.