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A microsomal ATP-activated pyridine nucleotide transhydrogenase
Authors:E P Hasson  C A West
Institution:Division of Biochemistry, Department of Chemistry, University of California, Los Angeles, California 90024 USA
Abstract:An ATP-activated transhydrogenase which catalyzes the reduction of TPN+ by DPNH has been demonstrated in the microsomal fraction from the endosperm of immature Echinocystis macrocarpa seeds. The activity is specifically dependent on the presence of ATP (Km of approximately 0.1 mm) of several nucleotides tested. The reaction is stimulated by MgCl2 addition up to concentrations of 6 mm. When 10?2m EDTA is added to the assay mixture in the absence of added MgCl2, a transhydrogenation reaction is observed which no longer shows any dependence on added ATP. A TPN+-dependent ATPase activity can be demonstrated in these preparations, but no fixed stoichiometry between ATP cleavage and TPNH formation could be established. A lag in attaining the maximal rate of transhydrogenation is seen unless the enzyme is preincubated for 10 min with ATP before initiating the reaction. It can further be shown that preincubation of the enzyme with ATP followed by removal of the ATP on a Dowex 1 column produces an enzyme capable of catalyzing the transhydrogenation without the further addition of ATP. 2,4-Dinitrophenol and thyroxin are effective inhibitors of the transhydrogenase and 2,4-dinitrophenol was shown to inhibit the activating effect of ATP during the preincubation period. It is concluded that the role of ATP is in the modification of the enzyme rather than direct participation in the transhydrogenation. The transhydrogenase is inhibited by ADP and AMP. This results in a response of the enzyme to adenylate energy charge in a manner characteristic for regulatory enzymes which participate in ATP-utilizing metabolic sequences.
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