| 仙鹤藓的孢子萌发及愈伤组织诱导 |
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| 引用本文： | 吴小凤,胡若洋,李学东. 仙鹤藓的孢子萌发及愈伤组织诱导[J]. 植物学通报, 2013, 0(6): 651-657 |
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| 作者姓名： | 吴小凤 胡若洋 李学东 |
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| 作者单位： | 首都师范大学生命科学学院,北京100048 |
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| 摘 要： | 通过对采自河北雾灵山海拔1500m的仙鹤藓（Atrichum undulatum）的孢子萌发以及原丝体发育的观察，发现仙鹤藓孢子无休眠现象，孢子接种3天左右萌发：其原丝体发育分为绿丝体和轴丝体两个阶段。扩大培养实验结果表明。仙鹤藓茎叶体在添加2％葡萄糖的MS培养基上，置于25℃／20℃、14小时光照/10小时黑暗、36μmol·m^-2·s^-1条件下培养．产生新生茎叶体最多，且茎叶体长势最好，可以获得大量无菌材料。仙鹤藓愈伤组织诱导实验显示，形成愈伤组织的最佳培养基为添加2％葡萄糖和1．0mg·L^-16-BA的MS培养基。
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| 关 键 词： | 仙鹤藓 愈伤组织 孢子萌发 |
Spore Germination and Callus Induction of Atrichum undulatum |
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| Xiaofeng Wu,Ruoyang Hu,Xuedong Li. Spore Germination and Callus Induction of Atrichum undulatum[J]. Chinese Bulletin of Botany, 2013, 0(6): 651-657 |
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| Authors: | Xiaofeng Wu Ruoyang Hu Xuedong Li |
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| Affiliation: | (School of Life Sciences, Capital Normal University, Beijing 100048, China) |
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| Abstract: | We studied the spore germination and protonema development of Atrichum undulatum collected from Wuling Mountain, Hebei Province （1 500 m a.s.l.）. The dormancy stage is absent during spore development. Spores in incubation culture take 3 days to germinate. Spores germinate exogenously by forming a protonema in 2 growth phases： chloronema and caulonema. The best growth conditions were MS medium with 2% glucose at 25℃/20℃, 14 h light/10 h dark, 36 IJmol·m^-2·s^-1. The optimal medium for callus induction is MS medium supplemented with 2% glucose and 1.0 mg·L^-1 6-BA to obtain high-quality cormus for large-scale cultivation. |
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| Keywords: | Atrichum undulatum callus spore germination |
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