Automatic whole broth multi-fermentor sampling |
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Authors: | K. D. Reda M. P. Thien I. Feygin C. S. Marcin M. M. Chartrain R. L. Greasham |
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Affiliation: | (1) Biochemical Process Research and Development, Merck, Sharp and Dohme Research Laboratories, Rahway, NJ, USA |
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Abstract: | Summary An efficient, aseptic method of obtaining whole broth fermentation samples was developed based on a piston-valve, a local sample loop, and an ability to drive the entire sample volume with sterile air through a sample line and into a remote tube. The configuration delivers 10-ml samples 10 m away with about 4 ml of broth wasted in the sampling process. An autosampler was enhanced and programmed to control acquisition into chilled tubes. The autosampler-based system represents a convenient way to provide frequent samples to profile intracellular and extracellular components for yeast and bacterial fermentations. A configuration to provide sampling from six fermentors with a multi-rack autosampler will be presented. |
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Keywords: | Fermentation monitoring Automatic sampling Aseptic sampling |
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