Anti-clotting activity of endothelial cell cultures and heparan sulfate proteoglycans |
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Authors: | P Colburn V Buonassisi |
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Institution: | Department of Biochemistry, University of California, Riverside, CA 91521 USA. |
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Abstract: | A photoaffinity probe for the vitamin D-dependent chick intestinal calcium binding protein (CaBP) has been prepared by conjugation of methyl-4-azidobenzoimidate (MABI) to lactoperoxidase-125I-iodinated CaBP to yield 125I-CaBP-MABI: 3 moles MABI per mole CaBP]. After incubation of 125I-CaBP-MABI (28,000 daltons) in model systems with bovine intestinal alkaline phosphatase (AP) (67,000 daltons), a UV light-dependent crosslinking occurred to yield a conjugate with a molecular weight of 95,000 (by SDS-gel electrophoresis); no crosslinking occurred with alkaline phosphatase. The formation of the 125I-CaBP-MABI-AP was found to occur only in the presence of calcium. |
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Keywords: | To whom all correspondence should be addressed |
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