Comparative Analysis of the Ribosomal Componentsof the Hydrogenosome-Containing Protist, Trichomonas vaginalis |
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Authors: | Nobuko Arisue Yasushi Maki Hideji Yoshida Akira Wada Lidya B Sánchez Miklós Müller Tetsuo Hashimoto |
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Institution: | (1) Department of Biosystems Science, Graduate University for Advanced Studies (Sokendai), Hayama, Kanagawa, 240-0193, Japan;(2) Department of Physics, Osaka Medical College, Takatsuki, Osaka, 569-0084, Japan;(3) The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA;(4) Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305-8572, Japan;(5) Present address: Department of Molecular Protozoology, Research Institute for Microbial Disease, Osaka University, Suita, Osaka, 565-0871, Japan;(6) Present address: Department of Biology, Faculty of Science, Niigata University, Niigata , 950-2181, Japan;(7) Present address: Public Health Research Institute, Newark, NJ 07103, USA |
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Abstract: | The ribosomes of the amitochondriate but hydrogenosome-containing protist lineage, the trichomonads, have previously been reported to be prokaryotic or primitive eukaryotic, based on evidence that they have a 70S sedimentation coefficient and a small number of proteins, similar to prokaryotic ribosomes. In order to determine whether the components of the trichomonad ribosome indeed differ from those of typical eukaryotic ribosomes, the ribosome of a representative trichomonad, Trichomonas vaginalis, was characterized. The sedimentation coefficient of the T. vaginalis ribosome was smaller than that of Saccharomyces cerevisiae and larger than that of Escherichia coli. Based on two-dimensional PAGE analysis, the number of different ribosomal proteins was estimated to be approximately 80. This number is the same as those obtained for typical eukaryotes (approximately 80) but larger than that of E. coli (approximately 55). N-Terminal amino acid sequencing of 18 protein spots and the complete sequences of 4 ribosomal proteins as deduced from their genes revealed these sequences to display typical eukaryotic features. Phylogenetic analyses of the five ribosomal proteins currently available also clearly confirmed that the T. vaginalis sequences are positioned within a eukaryotic clade. Comparison of deduced secondary structure models of the small and large subunit rRNAs of T. vaginalis with those of other eukaryotes revealed that all helices commonly found in typical eukaryotes are present and conserved in T. vaginalis, while variable regions are shortened or lost. These lines of evidence demonstrate that the T. vaginalis ribosome has no prokaryotic or primitive eukaryotic features but is clearly a typical eukaryotic type. |
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Keywords: | Ribosome Sedimentation coefficient Ribosomal protein rRNA Trichomonas vaginalis |
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