Aqueous two-phase extraction of 2,3-butanediol from fermentation broths by isopropanol/ammonium sulfate system |
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Authors: | Li-Hui Sun Bo Jiang Zhi-Long Xiu |
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Institution: | (1) Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian, 116024, People’s Republic of China |
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Abstract: | A novel aqueous two-phase system consisted of 2-propanol/ammonium sulfate was used for the extraction of 2,3-butanediol from
fermentation broths. The maximum partition coefficient and recovery of 2,3-butanediol reached 9.9 and 93.7%, respectively,
and more than 99% of the cells and about 85% of the soluble proteins were removed when 34% (w/w) 2-propanol and 20% (w/w)
ammonium sulfate were used. The separated cells could be re-used as inocula for subsequent fermentations. The aqueous two-phase
system described in this study may have potential application in the extraction of 2,3-butanediol produced by industrial fermentation
processes. |
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Keywords: | Aqueous two-phase extraction 2 3-Butanediol Cell recycle fermentation Isopropanol/ammonium sulfate |
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